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2-apb (ip3r inhibitor  (Millipore)


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    Structured Review

    Millipore 2-apb (ip3r inhibitor
    2 Apb (Ip3r Inhibitor, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ip3r+inhibitor+2-apb/xestospongin+c/10__1016_slash_j__fshw__2023__03__020-35-10-26
    Average 90 stars, based on 1 article reviews
    2-apb (ip3r inhibitor - by Bioz Stars, 2026-10
    90/100 stars

    Images

    Related Articles

    Quantitative RT-PCR:

    Article Title: HSV-1-induced disruption of transcription termination resembles a cellular stress response but selectively increases chromatin accessibility downstream of genes
    Article Snippet: The IP3R inhibitor 2-APB (100μM, Sigma-Aldrich), the PKC/PKD inhibitor Gö6976 (10μM; Tocris), the CaMKII inhibitor KN-93 (10μM; Tocris) and the calcium chelator BAPTA-AM (50μM; Cayman Chemical) were applied as described [ ].

    Article Title: A Novel Mechanism of Endoplasmic Reticulum Stress‐ and c‐Myc‐Degradation‐Mediated Therapeutic Benefits of Antineurokinin‐1 Receptor Drugs in Colorectal Cancer
    Article Snippet: Proteasome inhibitor MG132 from Solarbio Life Science (Beijing, China), MEK1/2 inhibitor U0126 and calcium chelating reagent BAPTA from Topscience Co., Ltd (Boston, USA), IP3R inhibitor 2-APB and DIDS (Sigma-Aldrich), 5-FU and SN-38 from MCE were dissolved in DMSO to 10 mM stock solution and stored at -20°C.

    Infection:

    Article Title: HSV-1-induced disruption of transcription termination resembles a cellular stress response but selectively increases chromatin accessibility downstream of genes
    Article Snippet: The IP3R inhibitor 2-APB (100μM, Sigma-Aldrich), the PKC/PKD inhibitor Gö6976 (10μM; Tocris), the CaMKII inhibitor KN-93 (10μM; Tocris) and the calcium chelator BAPTA-AM (50μM; Cayman Chemical) were applied as described [ ].

    Article Title: A Novel Mechanism of Endoplasmic Reticulum Stress‐ and c‐Myc‐Degradation‐Mediated Therapeutic Benefits of Antineurokinin‐1 Receptor Drugs in Colorectal Cancer
    Article Snippet: Proteasome inhibitor MG132 from Solarbio Life Science (Beijing, China), MEK1/2 inhibitor U0126 and calcium chelating reagent BAPTA from Topscience Co., Ltd (Boston, USA), IP3R inhibitor 2-APB and DIDS (Sigma-Aldrich), 5-FU and SN-38 from MCE were dissolved in DMSO to 10 mM stock solution and stored at -20°C.

    Cell Culture:

    Article Title: HSV-1-induced disruption of transcription termination resembles a cellular stress response but selectively increases chromatin accessibility downstream of genes
    Article Snippet: The IP3R inhibitor 2-APB (100μM, Sigma-Aldrich), the PKC/PKD inhibitor Gö6976 (10μM; Tocris), the CaMKII inhibitor KN-93 (10μM; Tocris) and the calcium chelator BAPTA-AM (50μM; Cayman Chemical) were applied as described [ ].

    Article Title: A Novel Mechanism of Endoplasmic Reticulum Stress‐ and c‐Myc‐Degradation‐Mediated Therapeutic Benefits of Antineurokinin‐1 Receptor Drugs in Colorectal Cancer
    Article Snippet: Proteasome inhibitor MG132 from Solarbio Life Science (Beijing, China), MEK1/2 inhibitor U0126 and calcium chelating reagent BAPTA from Topscience Co., Ltd (Boston, USA), IP3R inhibitor 2-APB and DIDS (Sigma-Aldrich), 5-FU and SN-38 from MCE were dissolved in DMSO to 10 mM stock solution and stored at -20°C.

    Expressing:

    Article Title: HSV-1-induced disruption of transcription termination resembles a cellular stress response but selectively increases chromatin accessibility downstream of genes
    Article Snippet: The IP3R inhibitor 2-APB (100μM, Sigma-Aldrich), the PKC/PKD inhibitor Gö6976 (10μM; Tocris), the CaMKII inhibitor KN-93 (10μM; Tocris) and the calcium chelator BAPTA-AM (50μM; Cayman Chemical) were applied as described [ ].

    Article Title: A Novel Mechanism of Endoplasmic Reticulum Stress‐ and c‐Myc‐Degradation‐Mediated Therapeutic Benefits of Antineurokinin‐1 Receptor Drugs in Colorectal Cancer
    Article Snippet: Proteasome inhibitor MG132 from Solarbio Life Science (Beijing, China), MEK1/2 inhibitor U0126 and calcium chelating reagent BAPTA from Topscience Co., Ltd (Boston, USA), IP3R inhibitor 2-APB and DIDS (Sigma-Aldrich), 5-FU and SN-38 from MCE were dissolved in DMSO to 10 mM stock solution and stored at -20°C.

    Activity Assay:

    Article Title: HSV-1-induced disruption of transcription termination resembles a cellular stress response but selectively increases chromatin accessibility downstream of genes
    Article Snippet: The IP3R inhibitor 2-APB (100μM, Sigma-Aldrich), the PKC/PKD inhibitor Gö6976 (10μM; Tocris), the CaMKII inhibitor KN-93 (10μM; Tocris) and the calcium chelator BAPTA-AM (50μM; Cayman Chemical) were applied as described [ ].

    Article Title: A Novel Mechanism of Endoplasmic Reticulum Stress‐ and c‐Myc‐Degradation‐Mediated Therapeutic Benefits of Antineurokinin‐1 Receptor Drugs in Colorectal Cancer
    Article Snippet: Proteasome inhibitor MG132 from Solarbio Life Science (Beijing, China), MEK1/2 inhibitor U0126 and calcium chelating reagent BAPTA from Topscience Co., Ltd (Boston, USA), IP3R inhibitor 2-APB and DIDS (Sigma-Aldrich), 5-FU and SN-38 from MCE were dissolved in DMSO to 10 mM stock solution and stored at -20°C.

    Positive Control:

    Article Title: HSV-1-induced disruption of transcription termination resembles a cellular stress response but selectively increases chromatin accessibility downstream of genes
    Article Snippet: The IP3R inhibitor 2-APB (100μM, Sigma-Aldrich), the PKC/PKD inhibitor Gö6976 (10μM; Tocris), the CaMKII inhibitor KN-93 (10μM; Tocris) and the calcium chelator BAPTA-AM (50μM; Cayman Chemical) were applied as described [ ].

    Article Title: A Novel Mechanism of Endoplasmic Reticulum Stress‐ and c‐Myc‐Degradation‐Mediated Therapeutic Benefits of Antineurokinin‐1 Receptor Drugs in Colorectal Cancer
    Article Snippet: Proteasome inhibitor MG132 from Solarbio Life Science (Beijing, China), MEK1/2 inhibitor U0126 and calcium chelating reagent BAPTA from Topscience Co., Ltd (Boston, USA), IP3R inhibitor 2-APB and DIDS (Sigma-Aldrich), 5-FU and SN-38 from MCE were dissolved in DMSO to 10 mM stock solution and stored at -20°C.

    Dot Blot:

    Article Title: HSV-1-induced disruption of transcription termination resembles a cellular stress response but selectively increases chromatin accessibility downstream of genes
    Article Snippet: The IP3R inhibitor 2-APB (100μM, Sigma-Aldrich), the PKC/PKD inhibitor Gö6976 (10μM; Tocris), the CaMKII inhibitor KN-93 (10μM; Tocris) and the calcium chelator BAPTA-AM (50μM; Cayman Chemical) were applied as described [ ].

    Article Title: A Novel Mechanism of Endoplasmic Reticulum Stress‐ and c‐Myc‐Degradation‐Mediated Therapeutic Benefits of Antineurokinin‐1 Receptor Drugs in Colorectal Cancer
    Article Snippet: Proteasome inhibitor MG132 from Solarbio Life Science (Beijing, China), MEK1/2 inhibitor U0126 and calcium chelating reagent BAPTA from Topscience Co., Ltd (Boston, USA), IP3R inhibitor 2-APB and DIDS (Sigma-Aldrich), 5-FU and SN-38 from MCE were dissolved in DMSO to 10 mM stock solution and stored at -20°C.

    Inhibition:

    Article Title: HSV-1-induced disruption of transcription termination resembles a cellular stress response but selectively increases chromatin accessibility downstream of genes
    Article Snippet: The IP3R inhibitor 2-APB (100μM, Sigma-Aldrich), the PKC/PKD inhibitor Gö6976 (10μM; Tocris), the CaMKII inhibitor KN-93 (10μM; Tocris) and the calcium chelator BAPTA-AM (50μM; Cayman Chemical) were applied as described [ ].

    Article Title: A Novel Mechanism of Endoplasmic Reticulum Stress‐ and c‐Myc‐Degradation‐Mediated Therapeutic Benefits of Antineurokinin‐1 Receptor Drugs in Colorectal Cancer
    Article Snippet: Proteasome inhibitor MG132 from Solarbio Life Science (Beijing, China), MEK1/2 inhibitor U0126 and calcium chelating reagent BAPTA from Topscience Co., Ltd (Boston, USA), IP3R inhibitor 2-APB and DIDS (Sigma-Aldrich), 5-FU and SN-38 from MCE were dissolved in DMSO to 10 mM stock solution and stored at -20°C.

    Purification:

    Article Title: HSV-1-induced disruption of transcription termination resembles a cellular stress response but selectively increases chromatin accessibility downstream of genes
    Article Snippet: The IP3R inhibitor 2-APB (100μM, Sigma-Aldrich), the PKC/PKD inhibitor Gö6976 (10μM; Tocris), the CaMKII inhibitor KN-93 (10μM; Tocris) and the calcium chelator BAPTA-AM (50μM; Cayman Chemical) were applied as described [ ].

    Article Title: A Novel Mechanism of Endoplasmic Reticulum Stress‐ and c‐Myc‐Degradation‐Mediated Therapeutic Benefits of Antineurokinin‐1 Receptor Drugs in Colorectal Cancer
    Article Snippet: Proteasome inhibitor MG132 from Solarbio Life Science (Beijing, China), MEK1/2 inhibitor U0126 and calcium chelating reagent BAPTA from Topscience Co., Ltd (Boston, USA), IP3R inhibitor 2-APB and DIDS (Sigma-Aldrich), 5-FU and SN-38 from MCE were dissolved in DMSO to 10 mM stock solution and stored at -20°C.



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    Image Search Results


    A. J and H cybrids pre-treated with an IP3R inhibitor, images show improved mitochondrial network and cellular distribution in J cybrids after 30 min of oxidative stress (20x). Scale bar = 10 μM. B. Higher magnification images (100x) show the structural changes before and after IP3Ri in stressed condition. (green: TOMM20; red: actin; blue: dapi; n = 6). Scale bar = 20 μM.

    Journal: Experimental eye research

    Article Title: Differential mitochondrial and cellular responses between H vs. J mtDNA haplogroup-containing human RPE transmitochondrial cybrid cells

    doi: 10.1016/j.exer.2022.109013

    Figure Lengend Snippet: A. J and H cybrids pre-treated with an IP3R inhibitor, images show improved mitochondrial network and cellular distribution in J cybrids after 30 min of oxidative stress (20x). Scale bar = 10 μM. B. Higher magnification images (100x) show the structural changes before and after IP3Ri in stressed condition. (green: TOMM20; red: actin; blue: dapi; n = 6). Scale bar = 20 μM.

    Article Snippet: IP3R inhibition/Flow cytometry 2-Aminoethoxydiphenyl borate (2-APB) (Santa Cruz Biotechnology, sc-201487) was used to inhibit the inositol 1,4,5-triphosphate receptor (IP3R).

    Techniques:

    A. The flow cytometry data showed that treatment with the IP3R inhibitor, 2-Aminoethoxydiphenyl borate (2APB) decreased [Ca2+]m under stressed conditions by 3.74-fold (n = 6; p < 0.001) in J cybrids as well as in H cybrids with 1.57-fold changes (n = 6; p < 0.001). B. Both RT and q-PCR showed that levels of expression of IP3R and MCU, which play a role in [Ca2+]m, are decreased by 1.60 and 1.51-fold (n = 3; p value; H is set at 1; ***p < 0.001). The brown bars show negative regulation, and the white bars show positive regulation.

    Journal: Experimental eye research

    Article Title: Differential mitochondrial and cellular responses between H vs. J mtDNA haplogroup-containing human RPE transmitochondrial cybrid cells

    doi: 10.1016/j.exer.2022.109013

    Figure Lengend Snippet: A. The flow cytometry data showed that treatment with the IP3R inhibitor, 2-Aminoethoxydiphenyl borate (2APB) decreased [Ca2+]m under stressed conditions by 3.74-fold (n = 6; p < 0.001) in J cybrids as well as in H cybrids with 1.57-fold changes (n = 6; p < 0.001). B. Both RT and q-PCR showed that levels of expression of IP3R and MCU, which play a role in [Ca2+]m, are decreased by 1.60 and 1.51-fold (n = 3; p value; H is set at 1; ***p < 0.001). The brown bars show negative regulation, and the white bars show positive regulation.

    Article Snippet: IP3R inhibition/Flow cytometry 2-Aminoethoxydiphenyl borate (2-APB) (Santa Cruz Biotechnology, sc-201487) was used to inhibit the inositol 1,4,5-triphosphate receptor (IP3R).

    Techniques: Flow Cytometry, Expressing

    FIGURE 3 | Effects of carbachol and 2-APB on HTV-induced Ca2+ homeostasis in lungs. Single-cell suspensions from lung tissues were preloaded with Fluo-4 AM, Rhod-2 and Mag-Fluo-AM, respectively. (A) Fluo-4 AM labeling the cytoplasmic Ca2+ in lung tissues from CON group, HTV group, IP3R agonist carbachol pretreatment upon HTV stimulation group (HTV+Carbachol group) and IP3R inhibitor 2-APB pretreatment upon HTV stimulation group (HTV+2-APB group). An inset picture was employed to show the indicated area at 4X magnification. (B) Fluorescence quantification analysis of Fluo-4 AM. (C) Rhod-2 and Mag-Fluo-AM co- dyeing to label Ca2+ in the mitochondria and ER in different groups. An inset picture was employed to show the indicated area at 4X magnification. (D) Fluorescence quantification analysis of Rhod-2. (E) Fluorescence quantification analysis of Mag-Fluo-AM. Data are expressed as means ± SD (n = 6 per group). *P < 0.05 vs. CON group; #P < 0.05 vs. HTV group.

    Journal: Frontiers in immunology

    Article Title: Inhibition of IP3R/Ca2+ Dysregulation Protects Mice From Ventilator-Induced Lung Injury via Endoplasmic Reticulum and Mitochondrial Pathways.

    doi: 10.3389/fimmu.2021.729094

    Figure Lengend Snippet: FIGURE 3 | Effects of carbachol and 2-APB on HTV-induced Ca2+ homeostasis in lungs. Single-cell suspensions from lung tissues were preloaded with Fluo-4 AM, Rhod-2 and Mag-Fluo-AM, respectively. (A) Fluo-4 AM labeling the cytoplasmic Ca2+ in lung tissues from CON group, HTV group, IP3R agonist carbachol pretreatment upon HTV stimulation group (HTV+Carbachol group) and IP3R inhibitor 2-APB pretreatment upon HTV stimulation group (HTV+2-APB group). An inset picture was employed to show the indicated area at 4X magnification. (B) Fluorescence quantification analysis of Fluo-4 AM. (C) Rhod-2 and Mag-Fluo-AM co- dyeing to label Ca2+ in the mitochondria and ER in different groups. An inset picture was employed to show the indicated area at 4X magnification. (D) Fluorescence quantification analysis of Rhod-2. (E) Fluorescence quantification analysis of Mag-Fluo-AM. Data are expressed as means ± SD (n = 6 per group). *P < 0.05 vs. CON group; #P < 0.05 vs. HTV group.

    Article Snippet: Reagents used in this study included IP3R agonist carbachol (Abmole Bioscience,USA), IP3R inhibitor 2-aminoethoxydiphenyl borate (2-APB, Tocris Biosciences, UK), and Ca2+ chelator September 2021 | Volume 12 | Article 729094 BAPTA-AM (MedChemExpress, USA).

    Techniques: Labeling, Fluorescence

    FIGURE 12 | Working model detailing the role of IP3R/Ca2+ in VILI. Mechanical overstretch induces cell inflammation through an accelerated ER-to-cytosolic and ER-to-mitochondria efflux of Ca2+ through IP3R, followed by ER stress and mitochondrial dysfunction.

    Journal: Frontiers in immunology

    Article Title: Inhibition of IP3R/Ca2+ Dysregulation Protects Mice From Ventilator-Induced Lung Injury via Endoplasmic Reticulum and Mitochondrial Pathways.

    doi: 10.3389/fimmu.2021.729094

    Figure Lengend Snippet: FIGURE 12 | Working model detailing the role of IP3R/Ca2+ in VILI. Mechanical overstretch induces cell inflammation through an accelerated ER-to-cytosolic and ER-to-mitochondria efflux of Ca2+ through IP3R, followed by ER stress and mitochondrial dysfunction.

    Article Snippet: Reagents used in this study included IP3R agonist carbachol (Abmole Bioscience,USA), IP3R inhibitor 2-aminoethoxydiphenyl borate (2-APB, Tocris Biosciences, UK), and Ca2+ chelator September 2021 | Volume 12 | Article 729094 BAPTA-AM (MedChemExpress, USA).

    Techniques: